Journal of the American Society for Mass Spectrometry

01 19 October 2026

DOI 10.1021/jasms.6c00300

Telescope: A Database Search Sandbox for Software Development

Open DOI

Michael R. Hoopmann, Jimmy K. Eng, Conor P. Herlihy et al.

Database search remains a cornerstone of data-dependent proteomics. Its adaptation to real-time search (RTS) enables intelligent, on-the-fly acquisition decisions such as triggering validation or quantitation scans only when warranted. B...

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02 18 October 2026

DOI 10.1021/jasms.6c00278

Mass Spectrometry-Based Footprinting Strategies for GPCR Structure Analysis

Open DOI

Ya T. Jin, Rory Roberts, Connor G. Smith et al.

G protein-coupled receptors (GPCRs) are a major class of membrane proteins that mediate diverse signaling pathways and represent the largest family of drug targets. However, their conformational flexibility and structural sensitivity to ...

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03 18 October 2026

DOI 10.1021/jasms.6c00288

A Novel Workflow to Assess Serum Stability of Antibody–siRNA Conjugates

Open DOI

Yihan Li, Lu Wang, Colin J. Phipps et al.

Antibody–siRNA conjugates (ARCs) are emerging as a promising approach for targeted delivery of small interfering RNA (siRNA) to specific cells outside the liver, harnessing the advantages of antibody-based delivery to overcome limitation...

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04 16 October 2026

DOI 10.1021/jasms.6c00385

Cytochrome c is Highly Compact upon Transfer into the Gas Phase from Strong Acids as Revealed by High-Pressure ESI Ion Mobility-Mass Spectrometry

Open DOI

Shunsuke Kamioka, Keijiro Ohshimo, Daiki Asakawa et al.

A substantial increase in the acidity of the protein solution (e.g., from pH 2 to pH 1) is known to induce the compaction of fully unfolded proteins, such as cytochrome c, into a non-native yet highly compact molten globule state, referr...

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05 14 October 2026

DOI 10.1021/jasms.6c00283

Asymmetric Track Lossless Analyzer Mass Spectrometry Improves Metaproteomic Analysis of a Complex Taxonomic Ground-Truth Microbial Community

Open DOI

Nicole M. Nightingale, Jana Richter, Nicolas Hartel et al.

Microbiomes, defined by communities of microorganisms, have emerged as significant contributors to broad areas of life, such as human health, agriculture, pollution remediation, and bioenergy production. While mass spectrometry (MS)-base...

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06 13 October 2026

DOI 10.1021/jasms.6c00287

Native Top-Down Mass Spectrometry Links Structure and Gas-Phase Stability of ERα-LBD

Open DOI

Kanchan Pathak, Emma C. Fink, Delaynie Brinkman et al.

Defining how protein assemblies form and dissociate is central to understanding biomolecular function. The ligand binding domain (LBD) of estrogen receptor α (ER) forms obligate head-to-head homodimers in solution. The high-affinity leuc...

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07 13 October 2026

DOI 10.1021/jasms.6c00178

Native Ion Mobility–Mass Spectrometry Reports Kinetically Trapped Conformational Ensembles, Not Single Structures

Open DOI

Christian Bleiholder, Fanny C. Liu

Native ion mobility/mass spectrometry (IM/MS) is widely used to characterize protein structures through their collision cross sections. This Perspective examines what structural information about proteins is contained in native IM/MS spe...

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08 12 October 2026

DOI 10.1021/jasms.6c00242

Advancing O-Glycosylation Analysis of Fc-Fusion Proteins at the Domain Level by HILIC-MS with Supercharging

Open DOI

Xiaojuan Li, Hongxia Wang, Ekaterina G. Deyanova

Fragment crystallizable (Fc)-fusion proteins are formed by the fusion of functional peptides or receptor extracellular domains (ECDs) with Fc. They are normally very heterogeneous with multiple N- and O-glycosylation sites and a high lev...

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09 8 October 2026

DOI 10.1021/jasms.6c00273

TherMIDAS: An Integrated Data Acquisition System for FT-ICR Mass Spectrometry

Open DOI

Jesse D. Canterbury, Michael W. Senko, Lydia Babcock-Adams et al.

Over 30 years ago, the Modular ICR Data Acquisition System (MIDAS) was assembled, developed, and deployed on the then-new Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) instruments at the National High Magnetic F...

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10 8 October 2026

DOI 10.1021/jasms.6c00253

IsoPairFinder: A Computational Workflow for Prioritizing LC–MS Feature Pairs Representing Candidate Microbial Pathway Intermediates from Stable Isotope Tracing Metabolomics

Open DOI

Zhiwei Zhou, Yuanyuan Liu, Mingxun Wang et al.

Functional annotation of microbial genes lags behind the pace of genome sequencing, leaving major gaps in our understanding of microbial metabolism. Combining genetic perturbation with untargeted stable isotope tracing metabolomics provi...

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